Xylanase from thermotolerant Bacillus haynesii strain, synthesis, characterization, optimization using Box-Behnken Design, and biobleaching activity

Abstract The economic value of xylanolytic enzymes is derived from their use in a variety industrial processes, which necessitates cost-effective manufacturing procedure. In the current study, forty bacterial isolates were isolated water samples and investigate efficacy to producing xylanase enzyme. most potent isolate was identified by sequencing amplifications 16Sr RNA gene as Bacillus haynesii strain K6. impacts various culture conditions on productivity xylane examined. Data showed that highest production achieved at pH 7, presence 3 g/L xylan, 5 peptone, incubated 40 °C for 24 h. Box-Behnken model used find best parameters relevant variables, results revealed an increase with values 35.02 U/mL. maximum precipitation optimized attained ammonium sulfate (60%) followed purification using dialysis sephadex G100 column chromatography. purified had 12-fold enrichment, specific activity 84 U/mg molecular weight approximately 439 KDa determined thin-layer chromatography (TLC)/mass spectrometry. amino acid analysis enzyme 15 acids, concentrations 1940 1520 mg/L proline cysteine, respectively. Finally, physical properties wastepaper pulp improved after treatment whiteness softness xylanase-treated percentages 34.6% 16.2%, Therefore, we recommend bleaching process it biologically synthetic material, safe, suitable use, reduces harmful chemicals.